LOCUS       40924_32480        8721 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pmT8 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Kuroda M, Kimizu M, Mikami C.
  TITLE     A simple set of plasmids for the production of transgenic plants
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (11), 2348-2351 (2010)
  PUBMED    21071849
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Kuroda M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2010) Contact:Masaharu Kuroda National 
            Agricultural Research Center, Rice Physiology Research Team; Inada 
            1-2-1, Joetsu, Niigata 943-0193, Japan
REFERENCE   3  (bases 1 to 8721)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8721)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "11"; 
            pages: "2348-2351"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAR-2010) Contact:Masaharu Kuroda National Agricultural Research
            Center, Rice Physiology Research Team; Inada 1-2-1, Joetsu, Niigata 
            943-0193, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..63
                     /label=T-DNA right border
                     /note="T-DNA right border"
     CDS             complement(1613..2260)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             2366..3562
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4841..5986)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6261..6629)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(6662..6771)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      6916..7625
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    7920..7944
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    complement(8656..8712)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 ttaattaaaa ctcattaatt gttgattttg tgatgactga tggcaggata tatgcggttg
       61 taattcattt ttattgtcta aatttctgta tttgtttgtt tgttcggttg taaatttttt
      121 tggaagccct atccaagttc atcatagcca gcttgaaggg aagggggcag cctgcaaatt
      181 gacggggaat gcttttgctg gcgtagggta tccgaggctc tttttgagct acacattgag
      241 atcgcgtgtc aggcggttga gggaacgttg cgatagccaa cgaggtcggt gtctccaggc
      301 atgaaacgtg gtatacgctt gttggcgatt tcaacaccgc gtttttacca tggcgcgctc
      361 ggatcgcagg accagctgcc ggcgccgatc tcctcccaag ccttggcaaa ttcagtgcct
      421 cgatcaaacg tggagatcgg gcgtggcata gacggcaagg gcgagaaggc agagattaca
      481 caactcatgg cttgagtact tgacccaaga ccaagaagag gatcgataga gtgaccatat
      541 aagtcgatat agcggcgatt gtcggatcga cttggtcgcg aagcgcactg accatctttc
      601 ggctcaaggt gcccccggta ccagcttcgg gctgaaattg caggacaact ggtgagtctg
      661 gatctgaagt tcattgttgt catggatgat ctggaccgac tggagccatc ccaggtggcg
      721 gaagtgttca ggcttgtgcg tgcagtagcc gatctgcccc gctttaccca tattctctgt
      781 tatgacaggc agattatcac tcatgccgtt gaacatgcgc tgaatatcga agatggcagc
      841 cgttatctcc agaaaatcat tcagcttagt tttaaattac cccgacctga agcctttgat
      901 ttacgtaatg aatttcgcca gcgggctgag gctctatatc agcaaattaa taatcaaccg
      961 ccagactctg gaatggtaag ggatctcatc gcggtgactg atacctatgg tgccgcactt
     1021 tcgacgccac gggaaatcca tcaggccatt aattccctga tttttcttta tccggggatg
     1081 cgggattttg tttatttccc tgatttgtgc ctgcttcagc ttatacgggt gacaaacccg
     1141 gctctgtatg actggacaga gcattacctg acagaacggt ccgtgattga aaccggtcag
     1201 ggtatgcttt ctgacggaga gaaagcagac ttccgggagg ggcttatcag atgtatgaag
     1261 acgttcaggg catcaaatgc agactcgttt ctgacacttg cagactggat ctatctcatc
     1321 tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac
     1381 gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag
     1441 aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa
     1501 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
     1561 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
     1621 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
     1681 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
     1741 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
     1801 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
     1861 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
     1921 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
     1981 ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt
     2041 cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg
     2101 cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca
     2161 accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca
     2221 gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga
     2281 taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca
     2341 gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg taattctgag
     2401 cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct
     2461 gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta
     2521 tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg
     2581 gcggccaatc ttgctcgtct cgctggccgg cgccactgtc gactacgcca tcatggcgac
     2641 agcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac
     2701 tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca
     2761 cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg
     2821 gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct
     2881 caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt
     2941 acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt
     3001 cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct
     3061 ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct
     3121 tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc
     3181 ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat
     3241 cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc
     3301 gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg
     3361 tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc
     3421 cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat
     3481 tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg
     3541 cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa
     3601 ggcgacttcc ggcaagctat acgcgcccta ggagtgcggt tggaacgttg gcccagccag
     3661 atactcccga tcacgagcag gacgccgatg atttgaagcg cactcagcgt ctgatccaag
     3721 aacaaccatc ctagcaacac ggcggtcccc gggctgagaa agcccagtaa ggaaacaact
     3781 gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc
     3841 aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca
     3901 aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca ggcggtaaag
     3961 gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa cgccatggaa
     4021 accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac
     4081 accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat caatcgtatc
     4141 gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac agcgcctacc
     4201 gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct ccagaatagc
     4261 gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt tggaatctgt
     4321 cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat accggcgacc
     4381 cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc
     4441 ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc gatgtaggcg
     4501 ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt tttctcctcg
     4561 tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa tcggatctcg
     4621 cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat cacaattgtc
     4681 aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact
     4741 gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg
     4801 ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     4861 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     4921 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     4981 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     5041 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     5101 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
     5161 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
     5221 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
     5281 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
     5341 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
     5401 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
     5461 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
     5521 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
     5581 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct
     5641 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
     5701 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg
     5761 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
     5821 gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg
     5881 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
     5941 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
     6001 ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg
     6061 ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc
     6121 cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc tccgcgaagt
     6181 cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt
     6241 tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg
     6301 ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg
     6361 aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc
     6421 aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt
     6481 ttgtagccct ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc
     6541 ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc
     6601 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
     6661 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
     6721 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
     6781 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
     6841 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
     6901 gaaaagatcc gtcgaccctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
     6961 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     7021 cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg
     7081 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     7141 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     7201 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     7261 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     7321 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     7381 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     7441 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg
     7501 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc
     7561 gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat
     7621 ggcagccaag ctggtaccgt gaacgtcggc tcgattgtac ctgcgttcaa atactttgcg
     7681 atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac ggatcgactg cttctctcgc
     7741 aacgccatcc gacggatgat gtttaaaagt cccatgtgga tcactccgtt gccccgtcgc
     7801 tcaccgtgtt ggggggaagg tgcacatggc tcagttctca atggaaatta tctgcctaac
     7861 cggctcagtt ctgcgtagaa accaacatgc aagctccacc gggtgcaaag cggcagcggc
     7921 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
     7981 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
     8041 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
     8101 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
     8161 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
     8221 atgccttgat ttcgccattc ccagataccc atttcatctt cagattggtc tgagattatg
     8281 cgaaaatata cactcatata cataaatact gacagtttga gctaccaatt cagtgtagcc
     8341 cattacctta cataattcac tcaaatgcta ggcagtctgt caactcggcg tcaatttgtc
     8401 ggccactata cgatagttgc gcaaattttc aaagtcctgg cctaacatca cacctctgtc
     8461 ggcggcgggt cccatttgtg ataaatccac catcacaata gatagtctaa tggacgaaaa
     8521 aggcgaatat ttcgatgctg agattcgacg caattaattc gagaaaaatc ccgtgattga
     8581 tgctgttgag ttaccaataa tatgggcagc gaaggccatt taattataag atccaaagcg
     8641 atcgcaaggc gcgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     8701 gagctcgaat tcttacgcgt t
//
